Validation of putative housekeeping genes for gene expression studies in human hepatocellular carcinoma using real-time quantitative RT-PCR
نویسندگان
چکیده
Validation of putative housekeeping genes for gene expression studies in human hepatocellular carcinoma using real-time quantitative RT-PCR. BMC Cancer 2008, 8:350 doi:10.1186/1471-2407-8-350 Vito R Cicinnati ([email protected]) Qingli Shen ([email protected]) Georgios C Sotiropoulos ([email protected]) Arnold Radtke ([email protected]) Guido Gerken ([email protected]) Susanne Beckebaum ([email protected])
منابع مشابه
Effect of valproic acid on SOCS1, SOCS3, JAK1, JAK2, STAT3, STAT5A, and SOCS5B in hepatocellular carcinoma HepG2 cell line
Background and aim: Aberrant activation of diverse intracellular signaling pathways involved in differentiation, cell growth, apoptosis. These pathways include known oncogenic pathways such as Janus kinase-signal transducer and activator of transcription (JAK/STAT) pathway. The JAK/STAT signaling pathway plays an important role in many cellular functions. This pathway can be activated by variou...
متن کاملEffects of 5-aza-2ˈ-deoxycytidine and Valproic Acid on Epigenetic-modifying DNMT1 Gene Expression, Apoptosis Induction and Cell Viability in Hepatocellular Carcinoma WCH-17 cell line
Background: DNA molecule of the eukaryotic cells is found in the form of a nucleoprotein complex named chromatin. Two epigenetic modifications are critical for transcriptional control of genes, including acetylation and DNA methylation. Hypermethylation of tumor suppressor genes is catalyzed by various DNA methyltransferase enzymes (DNMTs), including DNMT1, DNMT2, and DNMT3. The most well chara...
متن کاملValidation of a genus-specific gene; TPS, used as internal control in quantitative Real Time PCR of transgenic cotton
Identification of genes with invariant levels of gene expression is a prerequisite for validating transcriptomic changes accompanying development. Ideally expression of these genes should be independent of the morphogenetic process or environmental condition.We report here the validation of internal control gene i.e.TPS (trehalose 6-phosphate-synthase) in cotton (Gossypium spp), using TaqMan sy...
متن کاملComparative Analysis of the Effects of Valproic Acid and Tamoxifen on Proliferation, and Apoptosis of Human Hepatocellular Carcinoma WCH 17 CellLin
Background: Histone deacetylation of tumor suppressor genes such as estrogen receptor alpha (ERα) can induce cancer, which is reversible by epi-drugs such as valproic acid (VPA). The previous result indicated that tamoxifen (TAM) induced apoptosis in hepatocellular carcinoma (HCC). This study was designed to assess the apoptotic and antiproliferative effects of VPA and TAM and also the ef...
متن کاملEvaluation of reference genes for quantitative RT-PCR in Lolium temulentum under abiotic stress
Lolium temulentum L. (Darnel ryegrass) is a valuable model grass species for the study of stress in forage and turf grasses. Gene expression analysis by quantitative real-time RT-PCR relies on the use of proper internal standards. The aim of this studywas to identify and evaluate reference genes for use in real-time quantitative RT-PCR for abiotic stress studies in L. temulentum. Partial sequen...
متن کامل